Brandon_Lucas
Newbie
Posts: 2
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« on: August 07, 2011, 03:46:20 AM » |
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1. How does a genomic library differ from a cDNA library?
a. A genomic library consists of cloned DNA and a cDNA library consists of cloned RNA.
b. The genomic library contains only coding sequences and the cDNA library contains both coding and noncoding sequences.
c. The genomic library consists of double-stranded DNA and the cDNA library consists of single-stranded DNA.
d. A genomic library contains fragments from the entire genome of an organism and a cDNA library contains fragments representing only the genes that were transcribed in the cells from which mRNA was isolated.
e. A genomic library contains only noncoding sequences, whereas a cDNA library contains only coding sequences.
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2. Biotechnology has been revolutionized by the advent of DNA technology. This includes recombinant DNA technology, which among otther things, has enabled the following:
a. gene cloning to produce of multiple copies of a gene of interest
b. genetic engineering of organisms in order to alter their biological functions
c. production of large quantities of a protein of interest by cloning and expressing the gene that encodes it
d. A and B above
e. A, B and C above
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3. What is the most logical sequence of steps for cloning a gene of interest using a plasmid as the cloning vector, and inserting the recombinant plasmid into a bacterium?
I. Grow the bacteria on selective agar medium to identify the bacteria that were transformed with the recombinant plasmid.
II. Cut both DNA samples using restriction enzymes.
III. Extract plasmid DNA from bacterial cells and DNA from cells of the organism containing the gene of interest.
IV. Mix DNA samples to hydrogen-bond the ends of plasmid DNA fragments to those of nonplasmid DNA fragments, and add DNA ligase to join them together.
V. Transform bacteria with the DNA mixture.
a. III, II, IV, V, I
b. I, II, IV, III, V
c. II, III, V, IV, I
d. III, IV, V, I, II
e. IV, V, I, II, III
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4. Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
1. The sample is cooled to allow the primers to anneal (hybridize) to the target DNA.
2. The mixture is heated to a high temperature to denature (separate) the double-stranded target DNA.
3. Fresh DNA polymerase is added.
4. DNA polymerase adds nucletoides to the 3' ends of the primers (extension) to make two identical copies of the target DNA.
a. 3, 2, 1, 4
b. 1, 3, 2, 4
c. 3, 1, 4
d. 2, 3, 4
e. 2, 1, 4
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5.Which of the following methods may be used to introduce recombinant DNA into eukaryotic cells?
a. Electroporation, in which a brief electrical pulse creates temporary holes in the plasma membrane.
b. Microinjection, in which microscopically thin needles are used to inject the DNA.
c. Southern and northern blotting.
d. Only A and B are correct.
e. A, B, and C are correct.
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